Functional GLP-1R antibodies identified from a synthetic GPCR-focused library demonstrate potent blood glucose control

by Qiang Liu, Pankaj Garg, Burcu Hasdemir, Linya Wang, Emily Tuscano, Emily Sever, Erica Keane, Ana G Lujan Hernandez, Tom Z. Yuan, Eric Kwan, Joyce Lai, Greg Szot, Sreenivasan Paruthiyil, Fumiko Axelrod, Aaron K. Sato
Published 2021-01-01

Antibody blockade of CD96 by distinct molecular mechanisms

by Peter S. Lee, Bryant Chau, Ishita Barman, Christine Bee, Aarti Jashnani, Jason M. Hogan, Barbara Aguilar, Gavin Dollinger, Arvind Rajpal, Pavel Strop
Published 2021-01-01

A T cell redirection platform for co-targeting dual antigens on solid tumors

by Leonie Enderle, Karim H. Shalaby, Maryna Gorelik, Alexander Weiss, Levi L. Blazer, Marcin Paduch, Lia Cardarelli, Anthony Kossiakoff, Jarrett J. Adams, Sachdev S. Sidhu
Published 2021-01-01

Host cell protein detection gap risk mitigation: quantitative IAC-MS for ELISA antibody reagent coverage determination

by Daniel M. Waldera-Lupa, Yvonne Jasper, Pia Köhne, Ronja Schwichtenhövel, Heiner Falkenberg, Thomas Flad, Peter Happersberger, Bernd Reisinger, Alireza Dehghani, Roland Moussa, Thomas Waerner
Published 2021-01-01

Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeys

by Lise I. Loberg, Meha Chhaya, Alexander Ibraghimov, Edit Tarcsa, Andreas Striebinger, Andreas Popp, Lili Huang, Frank Oellien, Stefan Barghorn
Published 2021-01-01

Bispecific VH/Fab antibodies targeting neutralizing and non-neutralizing Spike epitopes demonstrate enhanced potency against SARS-CoV-2

by Shion A. Lim, Josef A. Gramespacher, Katarina Pance, Nicholas J. Rettko, Paige Solomon, Jing Jin, Irene Lui, Susanna K. Elledge, Jia Liu, Colton J. Bracken, Graham Simmons, Xin X. Zhou, Kevin K. Leung, James A. Wells
Published 2021-01-01

N-terminal selective conjugation method widens the therapeutic window of antibody–drug conjugates by improving tolerability and stability

by Min Ji Ko, Daehae Song, Juhee Kim, Jae Yong Kim, Jaehyun Eom, Byungje Sung, Yong-Gyu Son, Young Min Kim, Sang Hoon Lee, Weon-Kyoo You, Jinwon Jung
Published 2021-01-01

Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed b...

by Oksana Tyshchuk, Christoph Gstöttner, Dennis Funk, Simone Nicolardi, Stefan Frost, Stefan Klostermann, Tim Becker, Elena Jolkver, Felix Schumacher, Claudia Ferrara Koller, Hans Rainer Völger, Manfred Wuhrer, Patrick Bulau, Michael Mølhøj
Published 2019-10-01

Antibody Fc engineering for enhanced neonatal Fc receptor binding and prolonged circulation half-life

by Brian C. Mackness, Julie A. Jaworski, Ekaterina Boudanova, Anna Park, Delphine Valente, Christine Mauriac, Olivier Pasquier, Thorsten Schmidt, Mostafa Kabiri, Abdullah Kandira, Katarina Radošević, Huawei Qiu
Published 2019-10-01

Selection and characterization of FcεRI phospho-ITAM specific antibodies

by Nileena Velappan, Avanika Mahajan, Leslie Naranjo, Priyanka Velappan, Nasim Andrews, Nicholas Tiee, Subhendu Chakraborti, Colin Hemez, Tiziano Gaiotto, Bridget Wilson, Andrew Bradbury
Published 2019-10-01

Epitope mapping of anti-drug antibodies to a clinical candidate bispecific antibody

by Arthur J. Schick, Victor Lundin, Justin Low, Kun Peng, Richard Vandlen, Aaron T. Wecksler
Published 2022-12-01

CDR loop interactions can determine heavy and light chain pairing preferences in bispecific antibodies

by Monica L. Fernández-Quintero, Katharina B. Kroell, Lukas J. Grunewald, Anna-Lena M. Fischer, Jakob R. Riccabona, Klaus R. Liedl
Published 2022-12-01

TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life

by Emma Davé, Oliver Durrant, Neha Dhami, Joanne Compson, Janice Broadbridge, Sophie Archer, Asher Maroof, Kevin Whale, Karelle Menochet, Pierre Bonnaillie, Emily Barry, Gavin Wild, Claude Peerboom, Pallavi Bhatta, Mark Ellis, Matthew Hinchliffe, David P. Humphreys, Sam P. Heywood
Published 2023-12-01

Deciphering cross-species reactivity of LAMP-1 antibodies using deep mutational epitope mapping and AlphaFold

by Tiphanie Pruvost, Magali Mathieu, Steven Dubois, Bernard Maillère, Emmanuelle Vigne, Hervé Nozach
Published 2023-12-01

Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody

by Dario A. T. Cramer, Vojtech Franc, Anna-Katharina Heidenreich, Michaela Hook, Mahdi Adibzadeh, Dietmar Reusch, Albert J. R. Heck, Markus Haberger
Published 2023-12-01

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology

by Sarah-Jane Kellmann, Christian Hentrich, Mateusz Putyrski, Hanh Hanuschka, Manuel Cavada, Achim Knappik, Francisco Ylera
Published 2023-12-01

Strategies for clinical dose optimization of T cell-engaging therapies in oncology

by Kathryn Ball, Simon J Dovedi, Pavan Vajjah, Alex Phipps
Published 2023-12-01

Assessing developability early in the discovery process for novel biologics

by Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torelif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty, Andreas H. Laustsen
Published 2023-12-01

Current advances in biopharmaceutical informatics: guidelines, impact and challenges in the computational developability assessment of antibody therapeutics

by Rahul Khetan, Robin Curtis, Charlotte M. Deane, Johannes Thorling Hadsund, Uddipan Kar, Konrad Krawczyk, Daisuke Kuroda, Sarah A. Robinson, Pietro Sormanni, Kouhei Tsumoto, Jim Warwicker, Andrew C.R. Martin
Published 2022-12-01

Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6Rα to inhibit IL-6/ IL-6Rα/gp130 hexameric signaling complex formation

by Chun-Chi Chou, Kuo-Tai Hua, Min-Wei Chen, Chin-Jui Wu, Chun-Hua Hsu, Jann-Tay Wang, Michael Hsiao, Lin-Hung Wei
Published 2022-12-01