Ultra-Fast Impedimetric Immunoassay for Detection of <em>Streptococcus agalactiae</em> Using Carbon Electrode with Nanodiamonds Film

oleh: Daniel Bigus, Wioleta Lewandowska, Ewelina Bięga, Anna Grela, Aleksandra Siedlar, Marta Sosnowska, Magdalena Fabisiak, Tomasz Łęga, Yanina Dashkievich, Joanna Nowacka-Dośpiał, Katarzyna Palka, Sabina Żołędowska, Dawid Nidzworski

Format: Article
Diterbitkan: MDPI AG 2023-05-01

Deskripsi

This publication presents the results of work on the development of a quick and cheap electrochemical immunosensor for the diagnosis of infections with the pathogen <i>Streptococcus agalactiae</i>. The research was carried out on the basis of the modification of the well-known glassy carbon (GC) electrodes. The surface of the GC (glassy carbon) electrode was covered with a film made of nanodiamonds, which increased the number of sites for the attachment of anti-<i>Streptococcus agalactiae</i> antibodies. The GC surface was activated with EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide). Determination of electrode characteristics after each modification step, performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).