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The serum electrolytes, Sodium and Potassium are the important macronutrient to the human and it is supplied to the body via the solid and liquid food materials. These electrolytes have different roles in the body and these functions are crucial for life. Its rate of consumption and excretion may vary to the different geographical region, and this may cause the variable medical fitness. We investigated the serum & urinary profile of Na+ & K+, and compared it with their dietary intake of these macronutrients in 400 healthy subjects from two different regions, plain and hill. The subjects from the plain region consume more Na+ & K+ rather than hill region. The Serum & urinary profile of Na+ & K+ were observed higher in plain region and it may be the cause of their higher blood pressure as compared to the hill subjects.