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A simple method to fabricate antibacterial color-coated steel sheet was presented. The Ag-loaded TiO2 was well dispersed in steel coil coating coupled with some special additives, such as plasticizer, wetting dispersant, and flow agent, and finally became the part of coil coating without any negative influence on the properties of final products. The best process parameters were obtained by substantive trial experiments. Ag-loaded TiO2 with the addition of 2% (w/w) in steel coil coating not only improved antibacterial efficiency of the antibacterial color-coated sheet by reaching 99.99%, but also greatly increased the degradation percentage of methyl orange to 88% without decreasing physical properties. The antibacterial color-coated sheets are expected to be used as antimicrobial products in the construction industry considering its low cost and high effectiveness in inhibiting the growth of bacteria.