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<strong>Background</strong> PTEN is an anti-oncogene frequently inactivating in glioma. The previous study found that <em>PTEN</em> was closely related to cellular autophagy activity. The purpose of this paper is to study whether the inactivation of <em>PTEN</em> in glioma stem/progenitor cells (GSPCs) is correlative<br />with the low autophagic activity in GSPCs. <strong>Methods</strong> Wild-type <em>PTEN</em> genes were transferred into GSPCs mediated by adenovirus. The autophagic activity in GSPCs before or after the introduction of wild-type <em>PTEN</em> was detected by immunocytochemistry, electron microscopy, and Western blotting assay. <strong>Results</strong> After transfection of wild-type <em>PTEN</em>, a large number of microtuble-associated protein 1 light chain 3 (MAP1LC3)-positive granules could be found in the cytoplasm of GSPCs under a confocal microscopy, and these granules were demonstrated to be autophagosomes under an electron microscope. Moreover, the expression of autophagy-related gene <em>Beclin-1</em> significantly increased after the transfection of wild-type <em>PTEN</em> gene. <strong>Conclusion</strong> The inactivation of PTEN in GSPCs is one reason of the low autophagic activity of GSPCs. <br />