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CO2 huff and puff has been continuously considered as an import EOR method for tight oil reservoirs. However, the large number of evaluation parameters for reservoir screening has limited its application. This paper proposed a multi-index evaluation function for CO2 huff and puff method to facilitate the reservoir screening. By the means of orthogonal experimental design, it concludes the main controlling factors for screening a tight sand oil reservoir with multi-stage fractured horizontal wells. In addition, we built a calculation model for this multi-index evaluation function with Box-Benhken experimental design method. And this model has been successfully applied to screen wells for CO2 huff and puff of Y oilfield in Ordos.