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<p>We present the first description of the advertisement call of <em>Noblella lochites</em> based on recordings obtained at the Cordillera del Condor, province of Zamora-Chinchipe, Ecuador. The call of <em>N. lochites</em> has 6-8 pulsed notes of 369-537 ms, with a dominant frequency of 3.51-3.93 kHz. Variation in the number of pulsed notes in captive individuals suggests that <em>Noblella</em> frogs may have complex responses to stress situations.</p>