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The aim of this study is the evaluation of the sensitisation to molecular allergens of Alternaria alternata, Aspergillus fumigatus and Cladosporium herbarum in atopic dermatitis patients. The complete dermatological and allergological examination including the examination of the sensitisation to molecular allergens with Multiplex ISAC testing was performed in all included patients. The statistical evaluation of the relation between the sensitisation to Alt a 1, Alt a 6, Cla h 8, Asp f 1, Asp f 3, Asp f 6 and other molecular food and inhalant allergens was performed. The sensitisation to molecular fungal allergen was recorded altogether in 58% patients; it is in significant relation to the sensitisation to some food and inhallant molecular allergens, such as Peanut, Walnut, Hazelnut, Atlantic Cod, Black Tiger Shrimp, Apple, Kiwi, Peach, Celery, Timothy, London Plane tree, Cedar pollen allergen, Mugwort, Cat, and Horse.