Cryo-EM structure of a licensed DNA replication origin
oleh: Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-12-01 |
Deskripsi
Origins of replication are licensed by loading of MCM onto DNA, and origin firing depends on interaction with Cdc45 and GINS to form two CMG holo-helicases. Here, authors determine the cryo-EM structures of DNA-bound MCM and visualise a phospho-dependent MCM element important for Cdc45 recruitment.