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<p>During the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) expedition, meteorological conditions over the lowest 1 <span class="inline-formula">km</span> of the atmosphere were sampled with the DataHawk2 (DH2) fixed-wing uncrewed aircraft system (UAS). These in situ observations of the central Arctic atmosphere are some of the most extensive to date and provide unique insight into the atmospheric boundary layer (ABL) structure. The ABL is an important component of the Arctic climate, as it can be closely coupled to cloud properties, surface fluxes, and the atmospheric radiation budget. The high temporal resolution of the UAS observations allows us to manually identify the ABL height (<span class="inline-formula"><i>Z</i>_ABL</span>) for 65 out of the total 89 flights conducted over the central Arctic Ocean between 23 March and 26 July 2020 by visually analyzing profiles of virtual potential temperature, humidity, and bulk Richardson number. Comparing this subjective <span class="inline-formula"><i>Z</i>_ABL</span> with <span class="inline-formula"><i>Z</i>_ABL</span> identified by various previously published automated objective methods allows us to determine which objective methods are most successful at accurately identifying <span class="inline-formula"><i>Z</i>_ABL</span> in the central Arctic environment and how the success of the methods differs based on stability regime. The objective methods we use are the Liu–Liang, Heffter, virtual potential temperature gradient maximum, and bulk Richardson number methods. In the process of testing these objective methods on the DH2 data, numerical thresholds were adapted to work best for the UAS-based sampling. To determine if conclusions are robust across different measurement platforms, the subjective and objective <span class="inline-formula"><i>Z</i>_ABL</span> determination processes were repeated using the radiosonde profile closest in time to each DH2 flight. For both the DH2 and radiosonde data, it is determined that the bulk Richardson number method is the most successful at identifying <span class="inline-formula"><i>Z</i>_ABL</span>, while the Liu–Liang method is least successful. The results of this study are expected to be beneficial for upcoming observational and modeling efforts regarding the central Arctic ABL.</p>