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In this study, two methods for determination of formalin amount were applied on samples of inactivated vaccines representing local or foreign companies. The first method; matching method was conducted by using phenyl hydrazine while in the other method is; spectrophotometry phloroglucinol was used. Spectrophotometrical method was found more sensitive and more accurate than the matching one. At the same time, the spectrophotometry method could be used for determination of formalin in all inactivated vaccines either bacterial or viral vaccines.