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Background: Enteric opportunistic parasitic infections are the major source of diarrheal disease in developing countries mainly in Human Immunodeficiency virus (HIV) infected patients. Objective: The study was to detect enteric parasites causing diarrhea and their association with immune status in HIV-seropositive patients. Methods: The present study was conducted in tertiary care teaching Hospital, Baroda between January 2006 to January 2007 involving 100 Human Immunodeficiency virus (HIV) seropositive patients.From all patients stool samples collected and stool was examined for enteric parasites by microscopy with special staining methods. Results: A total of 100 HIV sero-positive patients with and without diarrhea were included in the study. Of the 100 patients, the protozoan parasitic infection was found in 28% (28/100). Out of 100 patients, 50 had diarrhea in which parasitic infection was 24 (48%) and 4 (4/50) protozoal parasites positive cases did not have diarrhea. A significant difference (p<0.05) was observed in the level of infection of intestinal protozoan between the HIV seropositive with diarrhea and HIV-seropositive without diarrhea. Conclusion: Enteric opportunistic parasitic infections were detected in 28% among HIV-seropositive patients. Early detection of enteric parasitic infections will help in the management and to improve the quality of life for HIV-infected individuals.