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Advances in whole-genome sequencing of many fungal species has revealed the presence of numerous &#8220;silent&#8221; biosynthetic genes, highlighting their potential to produce a wide variety of natural products. These silent biosynthetic genes are regulated in part by their highly condensed chromatin structure, which can be modified to allow transcription in response to external stimuli. In this study, <i>Asteromyces cruciatus</i> was subjected to both epigenetic modification and osmotic stress to enhance the production of new natural products. This &#8220;cooperative induction&#8221; strategy led to the isolation and characterization of two new polyketides from a fermentation of <i>A. cruciatus</i> treated with suberoylanilide hydroxamic acid and sodium chloride. The metabolic profiles of the control and treated samples were assessed using ultra-high performance liquid chromatography high-resolution electrospray ionization mass spectrometry (UHPLC-HRESIMS) metabolomic analysis, highlighting the upregulation of two new polyketides, primarolides A and B. These compounds were purified using reversed-phase flash chromatography followed by high-performance liquid chromatography, and their planar structures were established using NMR spectroscopy.