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Road maintenance in winter using de-icing agents, which is widely used in Eastern Europe, is the cause of water salinization in neighbouring environments, which might lead to biodiversity loss in aquatic ecosystems. In this study, we investigated NaCl toxicity to test young organisms: <i>Daphnia magna</i> and <i>Poecilla reticulata</i> (standard tests organisms). The salinity of NaCl was measured by electrolytic conductivity (EC). It was statistically demonstrated that the test solutions should be prepared using natural water. For <i>D. magna</i> the NOEC was 7.17 mS/cm and the LC50 9.76 mS/cm. <i>Poecilla reticulata</i> showed resistance to salinity up to a conductivity of 25.2 mS/cm, and no lethal effects were recorded for any individual in the test population. The study showed that winter salinities recorded in inland waters (without emergencies such as sudden influx of pollutants due to industrial accidents) are unlikely to affect fish but may be hazardous to small plankton. However, the high dare of <i>D. magna</i> may result in a reduction of planktivorous fish.