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Preconditioning (PC), is an approach to improve therapeutic potential of stem cells against ischemic environment. PC has several advantages over other therapeutic techniques as this results in increase of transplanted stem cells recruitment, retention, survival and subsequently the induction of a more supportive environment within the damaged tissue via secretion of angiogenic factors. Special attention is needed to recognize new materials, compounds, and conditions to assess the feasibility of PC for being applied in clinics to treat the ischemic diseases.