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Parthenocarpy and male sterility are highly desirable traits in tomato breeding and molecular study. The stamen carpelloid mutant generally displays male sterility. A natural mutant displaying <i>carpelloid stamen and parthenocarpy</i> (<i>csp</i>) was identified in our research group. In this study, the <i>csp</i> locus was finely mapped to a 65 kb interval, which contained six putative genes. One of them, <i>Solyc04g081000</i>, encodes the tomato class B MADS box gene <i>TAP3</i> (syn. <i>SlDEF</i>). Sequencing data revealed that a <i>copia</i> long terminal repeat retrotransposon was inserted in the first intron of the <i>TAP3</i> gene of the <i>csp</i> mutant. qRT-PCR showed that the expression of <i>TAP3</i> was significantly down-regulated in the petals and stamens of the <i>csp</i> mutant. A phenotypic analysis of the <i>TAP3</i> gene-edited mutants and allelism tests indicated that <i>TAP3</i> was the gene underlying <i>csp</i>, and <i>csp</i> was a novel allelic mutation of <i>TAP3</i>. The results of this study will lay the foundation for a further analysis of the function of <i>TAP3</i> and provide materials and a basis for a further study of the functional differentiation of tomato B-class genes.