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We sampled fish in pools located on tributaries of Cow Creek in the northern Sacramento Valley, and related fish distribution and habitat use to environmental factors across the 2003 agricultural growing season. This rangeland watershed experiences extensive livestock use, and many landowners divert stream water for pasture irrigation. Our goal was to provide landowners and managers with current baseline information about the conditions in which fish were found. Our results provide a basis for the development and comparison of irrigation best management practices that may improve conditions for native fish in rangeland streams.