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Bovine mastitis is an important disease of cattle which affects economy of the country. The purpose of study was isolation and identification of the common bacterial causes of clinical bovine mastitis. To this end, 50 bovine milk samples from CMT confirmed-clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar and Mac-Conkey agar plates for bacterial isolation. The major pathogens isolated were Escherichia coli (28%), Staphylococcus aureus (24%), Pseudomonas aeruginosa (18%), Klebsiella pneumoniae (10%) and Streptococcus sp. (2%) as per the biochemical tests and PCR. In conclusion, high percentage of positive samples and relatively high occurrence of environmental microorganisms indicates urgent need to emphisize udder hygiene for clean milk production.