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l-cystine hydrobromide (LCHBr) is a novel nonlinear optical material which has been synthesized and grown. The low-temperature solution growth technique was employed to grow the single crystal of LCHBr. The structure of the grown crystals was characterized by single-crystal XRD. The different functional groups of the grown LCHBr crystal are studied using FTIR spectroscopy. The transparent nature of the grown crystal was analysed by UV–Vis–NIR study. The mechanical strength of the grown crystal was analysed using microhardness study. The optical second harmonic generation conversion efficiency of the LCHBr crystal was studied using Nd:YAG laser. The laser damage threshold value of this grown crystal has been evaluated.