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• Background: Ultrasound is a noninvasive and accurate method for measurement of liver size and the evaluation of hepatic size is a daily question in abdominal ultrasound, especially to determine the presence of hepatomegaly. This study investigated a new method of ultrasound for liver span measurement with probe in contrast with previous methods using a ruler for calculating the distance between upper and lower liver margins. • Methods: Fifty nine patients with liver CT scan were evaluated with two ultrasound methods of probe and ruler liver span measurement and the findings were compared. • Finding: Ultrasound measurement of liver span with new method with probe had accuracy of 64.4% in cut of point of 10 mm in contrast with CT scan. • Conclusion: Probe method can be used as an accurate method instead of previous method of measurement with ruler.