Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction
oleh: Marize Pereira Miagostovich, Flávia Barreto dos Santos, Eliane Saraiva M de Araújo, Juarez Dias, Hermann G Schatzmayr, Rita Maria Ribeiro Nogueira
| Format: | Article |
|---|---|
| Diterbitkan: | Fundação Oswaldo Cruz (FIOCRUZ) 1997-09-01 |
Deskripsi
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas