Benchmarking common quantification strategies for large-scale phosphoproteomics
oleh: Alexander Hogrebe, Louise von Stechow, Dorte B. Bekker-Jensen, Brian T. Weinert, Christian D. Kelstrup, Jesper V. Olsen
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-03-01 |
Deskripsi
Quantitative phosphoproteomics has become a standard method in molecular and cell biology. Here, the authors compare performance and parameters of phosphoproteome quantification by LFQ, SILAC, and MS2-/MS3-based TMT and introduce a TMT-adapted algorithm for calculating phosphorylation site stoichiometry.