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The complete mitochondrial genome of Dabry’s sturgeon (Acipenser dabryanus) was first determined in this study. The mitochondrial DNA is circular molecule (16,437 bp in length) with a typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 1 non-coding control region (D-loop). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition of the heavy strain was A (30.2%), T (23.7%), C (29.7%), G (16.4%), and with an AT bias of 53.9%. Phylogenetic analysis based on the mitochondrial genomes of 10 related species using MEGA 5.1 indicated that A. dabryanus and A. sinensis are the most closely related species.