Find in Library

<i>CCT MOTIF FAMILY</i> (<i>CMF</i>) genes belong to the <i>CCT</i> gene family and have been shown to play a role in diverse processes, such as flowering time and yield regulation, as well as responses to abiotic stresses. <i>CMF</i> genes have not yet been identified in <i>Brassica rapa</i>. A total of 25 <i>BrCMF</i> genes were identified in this study, and these genes were distributed across eight chromosomes. Collinearity analysis revealed that <i>B. rapa</i> and <i>Arabidopsis thaliana</i> share many homologous genes, suggesting that these genes have similar functions. According to sequencing analysis of promoters, several elements are involved in regulating the expression of genes that mediate responses to abiotic stresses. Analysis of the tissue-specific expression of <i>BrCMF14</i> revealed that it is highly expressed in several organs. The expression of <i>BrCMF22</i> was significantly downregulated under salt stress, while the expression of <i>BrCMF5</i>, <i>BrCMF7</i>, and <i>BrCMF21</i> was also significantly reduced under cold stress. The expression of <i>BrCMF14</i> and <i>BrCMF5</i> was significantly increased under drought stress, and the expression of <i>BrCMF7</i> was upregulated. Furthermore, protein–protein interaction network analysis revealed that <i>A. thaliana</i> homologs of <i>BrCMF</i> interacted with genes involved in the abiotic stress response. In conclusion, <i>BrCMF5</i>, <i>BrCMF7</i>, <i>BrCMF14</i>, <i>BrCMF21</i>, and <i>BrCMF22</i> appear to play a role in responses to abiotic stresses. The results of this study will aid future investigations of <i>CCT</i> genes in <i>B. rapa</i>.