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Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property
oleh: Yanan Wang, Xuehong Chen, Xiaolin Bi, Yining Ren, Qi Han, Yu Zhou, Yantao Han, Ruyong Yao, Shangyong Li
Format: | Article |
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Diterbitkan: | MDPI AG 2019-05-01 |
Deskripsi
Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, <i>aly08</i>, was cloned from the marine bacterium <i>Vibrio</i> sp. SY01 and expressed in <i>Escherichia coli.</i> The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0−10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications.