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The objective of this research is the groundwater quality analysis in the Mygdonia basin (North Greece), where intensive agricultural activities take place. Groundwater sampling was carried out in May (17 samples) and in September (26 samples) for the years from 2013 to 2015. In situ physicochemical parameters (pH, EC, and T) were tested, whereas major ions (Ca²⁺, Na⁺, Mg²⁺, K⁺, NO₃⁻, SO₄²⁻, Cl⁻, and HCO₃⁻) were analyzed in the laboratory. Conventional statistical methods were applied for the classification of the groundwater hydrochemical type and the identification of the prevailing hydrogeological processes. Finally, some recommendations are presented for the protection of groundwater quality.