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Objective: Nanoparticles differ from the same material at larger scale in chemical and physical properties. The effect of nanoparticle on the blood cell still needs scientific verification. Material and Methods: The direct effect of gold nanoparticles on lymphocyte was direct assessed by in vitro assay. Results: In this work, the author reported additional results from specific observation on lymphocyte exposure to gold nanoparticle. Conclusion: This result confirms for direct intracellular penetration effect.