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Primulina ophiopogoides is a perennial herb of Gesneriaceae distributed on the limestone rocks. Here, the complete chloroplast (cp) genome of P. ophiopogoides was assembled and characterized. The cp genome is in a total length of 152,718 bp with the typical quadripartite structure, containing 2 inverted repeats (IRs) of 25,472 bp separated by a large single-copy (LSC) region of 83,615 bp and a small single-copy (SSC) region of 18,159 bp. The whole cp genome of P. ophiopogoides contains 131 genes, including 86 protein-coding genes, 37 tRNAs genes, and 8 rRNAs. The phylogenetic analysis indicated that P. ophiopogoides displayed a closer kinship to Primulina linearifolia.