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The present study reveals the incidence of infection of digenetic trematode, Dactylostomum ramgangaii, sp. nov. obtained from the small intestine of Mystus seenghala (sky) from the river Ramganga at Bareilly. The morphological studies reveals that these species differs from other known species in having only two dactyls on either side of ventral sucker, moderate pharynx, post bifurcal genital pore and in the shape of gonads. Dactylostomum elongatus sp. nov. has been obtained from intestine and body cavity of Mystus seenghala (sky) from the river Nakatia and local fish ponds. These species differs from other species on the basis of sessile ventral sucker with two dactyls lobed ovary and the vitelline follicles extending from pharynx to posterior and of the body.