Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
oleh: Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-11-01 |
Deskripsi
Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.