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The present investigation was taken to study the temperament score and its relation with various production and reproduction parameters in Vrindavani cows. Docile temperament was observed in 74.10% of the cows and restless temperament in 25.90%. The mean temperament scores were 1.92±0.03 and 2.27±0.04 for docile and restless animals, respectively. The least squares means significantly differed for heart girth and ratio of body weight to wither height in two temperament groups. However, the temperament score of cows had no significant effect on most of the production and reproduction traits, but the milking behavior traits were better for less temperament scored animals than higher one. Evaluating temperament score could be quite useful tool to the farmers as well as dairy producers in view of selection and management of dairy cattle for better productivity and profitability.