Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies
oleh: Anastasios D. Giannou, Birte Ohm, Dimitra E. Zazara, Jöran Lücke, Tao Zhang, Morsal Sabihi, Philipp Seeger, Jun Oh, Rainer Grotelüschen, Philipp Busch, Oliver Mann, Thilo Hackert, Jakob R. Izbicki, Yoshito Yamada, Samuel Huber, Wolfgang Jungraithmayr
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2023-12-01 |
Deskripsi
Summary: The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma.For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.