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Anthocyanins are responsible for the red color of strawberry, they are a subclass of flavonoids synthesized in cytosol and transferred to vacuole to form the visible color. Previous studies in model and ornamental plants indicated members of the glutathione S-transferase (<i>GST</i>) gene family were involved in vacuolar accumulation of anthocyanins. In the present study, a total of 130 <i>FaGST</i> genes were identified in the genome of cultivated strawberry (<i>Fragaria</i> × <i>ananassa</i>), which were unevenly distributed across the 28 chromosomes from the four subgenomes. Evolutionary analysis revealed the expansion of <i>FaGST</i> family was under stable selection and mainly drove by WGD/segmental duplication event. Classification and phylogenetic analysis indicated that all the <i>FaGST</i> genes were clarified into seven subclasses, among which <i>FaGST1</i>, <i>FaGST37</i>, and <i>FaGST97</i> belonging to Phi class were closely related to <i>FvRAP</i>, an anthocyanin-related <i>GST</i> of wildwood strawberry, and this clade was clustered with other known anthocyanin-related <i>GSTs</i>. RNAseq-based expression analysis at different developmental stages of strawberry revealed that the expression of <i>FaGST1</i>, <i>FaGST37</i>, <i>FaGST39</i>, <i>FaGST73</i>, and <i>FaGST97</i> was gradually increased during the fruit ripening, consistent with the anthocyanins accumulation. These expression patterns of those five <i>FaGST</i> genes were also significantly correlated with those of other anthocyanin biosynthetic genes such as <i>FaCHI</i>, <i>FaCHS</i>, and <i>FaANS</i>, as well as anthocyanin regulatory gene <i>FaMYB10</i>. These results indicated <i>FaGST1</i>, <i>FaGST37</i>, <i>FaGST39</i>, <i>FaGST73</i>, and <i>FaGST97</i> may function in vacuolar anthocyanin accumulation in cultivated strawberry.