Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis
oleh: Duangjai Nacapricha, Oraphan Anurukvorakun, Leena Suntornsuk, Orawon Chailapakul, Yasuaki Einaga, Piyada Jittangprasert, Kate Grudpan, Prapin Wilairat, Patcharin Chaisuwan, Thitirat Mantim, Chanpen Karuwan
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2006-01-01 |
Deskripsi
This work presents the construction and application of boron-doped diamond(BDD) thin film electrode as sensor for the determination of three β-agonists, viz.salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inertmaterial, BDD film however shows fouling in detection of these compounds using fixedpotentialmode amperometry. A suitable waveform for pulsed amperometric detection(PAD) was developed and used to determine the agonist compounds. It was seen that thedeveloped PAD significantly refreshed the BDD surface for long-term detection in flowinjection analysis. Linear working ranges were 0.5-100 μM, 1.0-100 μM and 0.5-50 μM forsalbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system wasapplied to successfully determine salbutamol and terbutaline in commercial pharmaceuticalproducts. The methods were validated with a capillary electrophoresis method.