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The present study will investigate the changes and correlation between the antioxidant enzyme superoxide dismutase (SOD) and the expression of interleukin 10 (IL-10) in rats fed a high-fat diet (HFD) after 6 weeks of aerobic exercise supplemented with the probiotic Lactobacillus bifidus. 40 male Wistar rats were divided into 5 groups (8 each): healthy control, high-fat diet (HFD), HFD+aerobic exercise (Tr), HFD+probiotic supplementation (Sup), and HFD+Tr+Sup. The rats in the HFD group received a special high-fat diet for 2 months prior to the main protocol. The rats in the exercise groups ran on a rodent treadmill for 6 weeks, 5 days a week. Lactobacillus bifidus supplementation was gavaged at a dose of 1 cc to the rats in the supplementation groups after each exercise session. Finally, the rats were sacrificed, and the IL-10 variable was measured using the Real-Time PCR method. The results of the present study indicated that the high-fat diet increased cholesterol and triglyceride levels (p<0.05). The HFD and HFD+Sup groups showed a significant decrease in SOD compared to the healthy control group (p<0.05). All treatment groups demonstrated a significant increase in SOD compared to the HFD group (p<0.05). The HFD+Tr and HFD+Tr+Sup groups showed a significant increase in IL-10 compared to the HFD group (p<0.05). However, the correlation between SOD and IL-10 was not confirmed (p>0.05). There was no correlation between the antioxidant enzyme and IL-10. Nevertheless, exercise and Lactobacillus bifidus were able to regulate the lipid profile, SOD, and IL-10 after a high-fat diet.