Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
oleh: Fuqiang Chen, Xiao Ding, Yongmei Feng, Timothy Seebeck, Yanfang Jiang, Gregory D. Davis
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-04-01 |
Deskripsi
Bacteria possess a diverse collection of CRISPR-Cas systems but many do not function in mammalian cells, precluding their use in genome editing. Here the authors show that by manipulating local chromatin structures, inhibited Cas9s can have their activity restored in human cells.