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Objective: The present study aimed to evaluate the bond strength of Sure-Seal Root as a new bioceramic-based sealer, and compare it with other sealers, including an epoxy resin-based sealer (AH-Plus), zinc oxide eugenol (ZOE) and a mineral trioxide aggregate-based sealer (MTA Fillapex).Methods: In this in vitro study, 40 extracted mandibular premolars were randomly assigned into 4 groups (n=10) according to the type of sealer applied as follows: Group 1: AH-Plus, Group 2: MTA-Fillapex, Group 3:  Sure-Seal Root, and Group 4: ZOE. The canals were prepared and obturated with gutta-percha and the corresponding sealer. The samples were sectioned into horizontal segments, and the push-out bond strength was determined using a universal testing machine at the coronal, middle, and apical root thirds. The data were analyzed by repeated measures ANOVA, and the significance level was set at P<0.05.Results: There were no significant differences in the push-out bond strength between groups in the coronal third (P>0.05). In the middle third, AH-Plus exhibited significantly greater bond strength compared to Sure-Seal Root and ZOE sealers (P<0.05), whereas MTA-Fillapex was not significantly different from the other groups (P>0.05). In the apical third, both AH-Plus and MTA-Fillapex showed significantly greater push-out bond strength than Sure-Seal Root and ZOE sealers (P<0.05).Conclusions: AH-Plus sealer exhibited the highest and ZOE showed the lowest bond strength. Sure-Seal Root indicated promising bond strength results when compared to ZOE and MTA-Fillapex. The push-out bond strength of all sealers to dentin increased from the coronal to the apical third.