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Objective To investigate effect of Duraphat on preventing enamel demineralization during orthodontic treatment.Methods 58 patients underwent fixed orthodontic treatment were included. Maxillary teeth were divided into 2 groups at central incisors. The teeth on one side were randomly assigned as experimental group and the contralateral teeth were control group. After bonding, the teeth of experimental group were applied with Duraphat after brackets being bonded, while control group received no treatment. Duraphat was applied every 3 months. The occurrence of enamel demineralization was observed and documented in both experimental and control groups before treatment and in 6th and 12th month during treatment, and the enamel decalcification index was also calculated.Results With the extension of treatment time, enamel demineralization of control group increased gradually. While that of experimental group is no obvious. The rate and severity of enamel demineralization in experimental side were significantly lower than those of the control side (P<0.05).Conclusion Duraphat has effect on the prevention of enamel demineralization during fixed orthodontic treatment.