Structural insights into DNA cleavage activation of CRISPR-Cas9 system
oleh: Cong Huai, Gan Li, Ruijie Yao, Yingyi Zhang, Mi Cao, Liangliang Kong, Chenqiang Jia, Hui Yuan, Hongyan Chen, Daru Lu, Qiang Huang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-11-01 |
Deskripsi
CRISPR-Cas9 is widely used for genome engineering but structural data for the DNA cleavage step are still incomplete. Here, the authors present the cryo-EM structure of a ternary Cas9-sgRNA-target DNA complex, perform MD simulations and discuss implications for the Cas9 DNA cleavage mechanism.