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<p>Abstract</p> <p>Background</p> <p>Glucose repression of transcription in the yeast, <it>Saccharomyces cerevisiae,</it> has been shown to be controlled by several factors, including two repressors called Mig1 and Mig2. Past results suggest that other repressors may be involved in glucose repression.</p> <p>Results</p> <p>By a screen for factors that control transcription of the glucose-repressible <it>SUC2</it> gene of <it>S. cerevisiae,</it> the <it>NRG1</it> gene was identified. Analysis of an <it>nrg1Δ</it> mutant has demonstrated that mRNA levels are elevated at both the <it>SUC2</it> and of the <it>GAL</it> genes of <it>S. cerevisiae</it> when cells are grown under normally glucose-repressing conditions. In addition, genetic interactions have been detected between <it>nrg1Δ</it> and other factors that control <it>SUC2</it> transcription.</p> <p>Conclusions</p> <p>The analysis of <it>nrg1Δ</it> demonstrates that Nrg1 plays a role in glucose repression of the <it>SUC2</it> and <it>GAL</it> genes of <it>S. cerevisiae.</it> Thus, three repressors, Nrg1, Mig1, and Mig2, are involved as the downstream targets of the glucose signaling in S. cerevisiae.</p>