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Travel training programs that include components of the real environment, simulation, application, and virtual reality may facilitate increased use of fixed-route public transport among people with disabilities (PWD). Existing evidence on travel training programs focused on outcomes in youth with disabilities. However, little is known about how such programs may influence knowledge and psychosocial skills for public transport use among adult with disabilities and older adults. This systematic review aims to examine the influence of travel training on knowledge, psychosocial skills (e.g., transportation-related self-efficacy and self-determination), and public transport use among adult with disabilities and older adults. This protocol of systematic review will be guided by the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) 2015. The literature search will include empirical studies published in French and English from 1990 to 2023 in the following database: MEDLINE, PsycINFO, and Transport Database via Ovid platform, and Embase and the Web of science. An update will be made to ensure that all relevant material is retrieved. All literature search results will be imported in Covidence software for screening and data extraction. Given that we expect to have studies with a variety of data collection methods and tools, the meta-analysis will not be used. The results of this review will be reported according to PRISMA 2020. A review of the influence of travel training on various outcomes will facilitate transport operators to develop and implement the best travel training interventions to better meet public transit needs of PWD.