Find in Library

Objective To identify and analyze a case of ABO discrepancy between forward and reverse blood grouping, and to provide reference for the identification of ambiguous blood group in clinical. Methods ABO and Rh blood group typing, absorption and elution test, and gene sequencing were performed to confirm the ambiguous blood group. Results The sample was identified by absorption and elution test and molecular biological method to be Ael subtype, and was named ABO*AEL.05/ABO*O.01.01 by ISBT. After family investigation, the proband and her second son share the same characteristic mutation site, and was named ABO*AEL.05/ABO*B.01.01 by ISBT. Conclusion Multiple blood group serological tests and molecular biology tests help to identify ABO subtypes, thus assuring the safety, scientificity and rationality of clinical blood transfusion.