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Phage Therapy against <em>Staphylococcus aureus</em>: Selection and Optimization of Production Protocols of Novel Broad-Spectrum <em>Silviavirus</em> Phages
oleh: Camille Kolenda, Mathieu Medina, Mélanie Bonhomme, Floriane Laumay, Tiphaine Roussel-Gaillard, Patricia Martins-Simoes, Anne Tristan, Fabrice Pirot, Tristan Ferry, Frédéric Laurent, PHAGEinLYON Study Group
Format: | Article |
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Diterbitkan: | MDPI AG 2022-09-01 |
Deskripsi
<b>Background:</b> Phage therapy a promising antimicrobial strategy to address antimicrobial resistance for infections caused by the major human pathogen <i>Staphylococcus aureus</i>. Development of therapeutic phages for human use should follow pharmaceutical standards, including selection of strictly lytic bacteriophages with high therapeutic potential and optimization of their production process. <b>Results:</b> Here, we describe three novel <i>Silviavirus</i> phages active against 82% of a large collection of strains (n = 150) representative of various methicillin-susceptible and -resistant <i>S. aureus</i> clones circulating worldwide. We also investigated the optimization of the efficiency and safety of phage amplification protocols. To do so, we selected a well-characterized bacterial strain in order to (i) maximize phage production yields, reaching phage titres of 10<sup>11</sup> PFU/mL in only 4 h; and (ii) facilitate phage purity while minimizing the risk of the presence of contaminants originating from the bacterial host; i.e., secreted virulence factors or induced temperate phages. <b>Conclusions:</b> In sum, we propose a quality-by-design approach for the amplification of broad-spectrum anti-<i>S. aureus</i> phages, facilitating the subsequent steps of the manufacturing process; namely, purification and quality control.