Generation of iPSC lines from primary human amniotic fluid cells
oleh: Katharina Drews, Peggy Matz, James Adjaye
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2015-11-01 |
Deskripsi
By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.