Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

oleh: Luana Gabriela Ferreira dos Santos, Andréia Lima Tomé Melo, Jonas Moraes-Filho, Rute Witter, Marcelo Bahia Labruna, Daniel Moura de Aguiar

Format: Article
Diterbitkan: Colégio Brasileiro de Parasitologia Veterinaria

Deskripsi

The present study evaluated the presence of EhrlichiaDNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsbgene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.