Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate
oleh: Ferdos Abid Ali, Ludovic Renault, Julian Gannon, Hailey L. Gahlon, Abhay Kotecha, Jin Chuan Zhou, David Rueda, Alessandro Costa
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-02-01 |
Deskripsi
The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during replication, a process that requires the ATPase-dependent activity of the MCM complex. Using cryo-EM reconstructions of the CMG complex in different conformations, the authors propose a model where the N-terminal and AAA+ domains of MCM work in concert to translocate along DNA.