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Abstract Background Workplace violence (WPV) has been recognized as a major occupational hazard worldwide. Healthcare professions are particularly at a higher risk of WPV. Patients and their relatives are commonly the most common perpetrators for WPV against physicians. Trainings on the universal precautions of violence, how to effectively anticipate, recognize and manage potentially violent situation is recommended by OSHA as a part of a written, effective, comprehensive, and interactive WPV prevention program. Objective To implement and evaluate the effectiveness of a training session delivered to nurses. The training session aimed to increase nurses’ ability to identify potentially violent situations and to effectively manage these situations in a teaching hospital in Egypt. Methodology A total of 99 nurses attended the training sessions. Confidence in coping with aggressive patient scale, along with nurses’ attitudes toward WPV, were used to assess the effectiveness of the training sessions. Results Nurses’ perceived confidence to deal with aggression increased after attending the training sessions. Nurses’ attitudes toward WPV positively changed after attending the training session. Conclusion and recommendations Increasing awareness of the problem among healthcare professions as well as the public is warranted. Violence prevention program with a zero-tolerance policy is warranted.