An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae
oleh: Bingyin Peng, Lygie Esquirol, Zeyu Lu, Qianyi Shen, Li Chen Cheah, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-05-01 |
Deskripsi
Gene dosage-based expression upregulation suffers from instability and random gene integration. Here, the authors report HapAmp, a method that uses haploinsufficiency as evolutionary force to drive in vivo gene amplification, and demonstrate its applications in protein and biochemical production in yeast.