CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus
oleh: Patricia A. Clow, Menghan Du, Nathaniel Jillette, Aziz Taghbalout, Jacqueline J. Zhu, Albert W. Cheng
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-04-01 |
Deskripsi
Three-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Here the authors present a CRISPR-based approach for labeling the genome at multiple nonrepetitive loci in living cells and to image chromatin loops in the presence and absence of cohesin.