Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A
oleh: Timm Fiebig, Johannes T. Cramer, Andrea Bethe, Petra Baruch, Ute Curth, Jana I. Führing, Falk F. R. Buettner, Ulrich Vogel, Mario Schubert, Roman Fedorov, Martina Mühlenhoff
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-09-01 |
Deskripsi
Neisseria meningitidis capsular polysaccharide (CPS) is a major virulence factor and vaccine formulations against Neisseria meningitidis serogroup A (NmA) contain O-acetylated CPS. Here, the authors provide mechanistic insights into CPS O-acetylation in NmA by determining the crystal structure of the O-acetyltransferase CsaC and NMR measurements further reveal that the CsaC-mediated reaction is regioselective for O3 and that the O4 modification results from spontaneous O-acetyl migration.